Article
Establishing primary cultures of vascular smooth muscle cells from the spiral modiolar artery.
International journal of pediatric otorhinolaryngology - 1 Aug 2012
Wang Ye, Qiao Li, Qiu Jianhua, Mi Wenjuan, Han Yu, Zhong Cuiping
Abstract excerpt
OBJECTIVE: This article is reporting a method for establishment primary cultures of vascular smooth muscle cells (VSMCs) from the spiral modiolar artery (SMA). METHODS: VSMCs were isolated from guinea pig SMAs. Arterial tissues were cut and enzymatically digested at 37 °C for 20 min using a 0.1% trypsin solution. After digestion, tissue fragments were explanted in a 35-mm culture dish. Contaminated fibroblasts...
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