How to infer reliable diploid genotypes from NGS or traditional sequence data: from basic probability to experimental optimization.

doi:10.1111/j.1420-9101.2012.02488.x

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Article

How to infer reliable diploid genotypes from NGS or traditional sequence data: from basic probability to experimental optimization.

Journal of evolutionary biology - 1 May 2012

Chenuil A

Abstract excerpt

The use of diploid sequence markers is still challenging despite the good quality of the information they provide. There is a common problem to all sequencing approaches [traditional cloning and sequencing of PCR amplicons as well as next-generation sequencing (NGS)]: when no variation is found within the sequences from a given individual, homozygozity can never be asserted with certainty. As a consequence,...

Topics

Alleles
Bayes Theorem
Biomarkers
Cloning, Molecular
Databases, Genetic
Diploidy
Genetic Loci
Genetics, Population
Genotyping Techniques
Heterozygote
Homozygote
Models, Genetic
Mutation
Reproducibility of Results
Sequence Analysis, DNA

Source

Open in PubMed

Identifiers

PMID: 22420488
DOI: 10.1111/j.1420-9101.2012.02488.x

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