Article
Identification of a novel mutation in FAD2B from a peanut EMS mutant with elevated oleate content.
Journal of oleo science - 1 Jan 2012
Fang Chao Qi, Wang Chuan Tang, Wang Pi Wu, Tang Yue Yi, Wang Xiu Zhen, Cui Feng Gao, Yu Shan Lin
Abstract excerpt
A Virginia type peanut mutant with more than 60% oleate content (E2-4-83-12) was selected from an EMS mutagenized population of LF2 (an export type peanut cultivar with 44.2% oleate) by near infrared reflectance spectroscopy. Cloning and sequencing of FAD2B from LF2 and E2-4-83-12 identified a novel mutation (C313T in the coding region) causing an H105Y substitution in the first histidine box of the FAD2B...
Topics
- Agriculture
- Amino Acid Sequence
- Arachis
- Base Sequence
- Ethyl Methanesulfonate
- Fatty Acid Desaturases
- Gas Chromatography-Mass Spectrometry
- Genes, Plant
- Molecular Sequence Data
- Mutagenesis
