Article
Coverage-based consensus calling (CbCC) of short sequence reads and comparison of CbCC results to identify SNPs in chickpea (Cicer arietinum; Fabaceae), a crop species without a reference genome.
American journal of botany - 1 Feb 2012
Azam Sarwar, Thakur Vivek, Ruperao Pradeep, Shah Trushar, Balaji Jayashree, Amindala BhanuPrakash, Farmer Andrew D, Studholme David J, May Gregory D, Edwards David, Jones Jonathan D G, Varshney Rajeev K
Abstract excerpt
PREMISE OF THE STUDY: Next-generation sequencing (NGS) technologies are frequently used for resequencing and mining of single nucleotide polymorphisms (SNPs) by comparison to a reference genome. In crop species such as chickpea (Cicer arietinum) that lack a reference genome sequence, NGS-based SNP discovery is a challenge. Therefore, unlike probability-based statistical approaches for consensus calling and by...
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