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Article

Evaluation of Differentiated Human Bronchial Epithelial Cell Culture Systems for Asthma Research

11 Jan 2012

Abstract excerpt

The aim of the current study was to evaluate primary (human bronchial epithelial cells, HBEC) and non-primary (Calu-3, BEAS-2B, BEAS-2B R1) bronchial epithelial cell culture systems as air-liquid interface- (ALI-) differentiated models for asthma research. Ability to differentiate into goblet (MUC5AC+) and ciliated (β-Tubulin IV+) cells was evaluated by confocal imaging and qPCR. Expression of tight...

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PMID: 22287976
DOI: 10.1155/2012/943982

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