Article
High efficiency of reprogramming CD34⁺ cells derived from human amniotic fluid into induced pluripotent stem cells with Oct4.
Stem cells and development - 10 Aug 2012
Liu Te, Zou Gang, Gao Yongtao, Zhao Xinrong, Wang Hui, Huang Qin, Jiang Lizhen, Guo Lihe, Cheng Weiwei
Abstract excerpt
Although many techniques can be used to generate multitype-induced pluripotent stem (iPS) cells from multitype seed cells, improving the efficiency and shortening the period of cell reprogramming remain troublesome issues. In this study, to generate iPS cells, CD34⁺ cells, isolated from human amniotic fluid cells (HuAFCs) by flow cytometry, were infected with retroviruses carrying only one reprogramming factor...
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