Article
[PCR-based sequencing for detection and genotyping of human papillomavirus in 325 cervical cell specimens from Beijing area].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Oct 2011
Cai Yupin, Zhang Ruifen, Li Yuan, Xia Xiaoyu, Xiang Yang, Zhu Baoli, Yang Yi
Abstract excerpt
To evaluate PCR-sequencing for clinical detection of human papillomavirus (HPV) genotypes in cervical cell specimens, we applied PCR-sequencing to HPV detection and genotyping by general primer PGMY09/11, which targets the HPV most conserved L1 gene. Samples with multiple infections were subjected to HPV type-specific PCR. Among the 325 cervical samples, 228 were HPV positive, of which 66 showed multiple...
Topics
- Adult
- Aged
- Cervix Uteri
- China
- Female
- Genotype
- Human papillomavirus 16
- Humans
- Middle Aged
- Papillomaviridae
- Papillomavirus Infections