Article
Generation of a human urinary bladder smooth muscle cell line.
In vitro cellular & developmental biology. Animal - 1 Feb 2012
Zheng Yongmu, Chang Shaohua, Boopathi Ettickan, Burkett Sandra, John Mary, Malkowicz S Bruce, Chacko Samuel
Abstract excerpt
We report a cell line (hBSM) established from human urinary bladder wall smooth muscle that maintains most of the phenotypic characteristics of smooth muscle cells. Cells were dissociated from the muscular layer with collagenase (1 mg/ml) and collected and grown in M199 supplemented with 10% fetal calf serum and 1% antibiotic-antimycotic. Primary cultures were grown for 2 d and small colonies were isolated by...
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