Reaction energetics of a mutant 3-oxo-delta 5-steroid isomerase with an altered active site base (D38E).

doi:10.1021/bi00250a044

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Article

Reaction energetics of a mutant 3-oxo-delta 5-steroid isomerase with an altered active site base (D38E).

Biochemistry - 22 Nov 1994

Zawrotny M E, Pollack R M

Abstract excerpt

3-Oxo-delta 5-steroid isomerase (KSI) catalyzes the isomerization of a variety of 3-oxo-delta 5-steroids to their conjugated delta 4-isomers through the formation of an intermediate dienol. Mutation of the catalytic base (Asp-38) to Glu (D38E) has been found to reduce kcat/Km for the isomerizatio...

Topics

Aspartic Acid
Binding Sites
Catalysis
Glutamic Acid
Kinetics
Methanol
Mutation
Steroid Isomerases
Thermodynamics

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