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Article

Reaction energetics of a mutant 3-oxo-delta 5-steroid isomerase with an altered active site base (D38E).

Biochemistry - 22 Nov 1994

Zawrotny M E, Pollack R M

Abstract excerpt

3-Oxo-delta 5-steroid isomerase (KSI) catalyzes the isomerization of a variety of 3-oxo-delta 5-steroids to their conjugated delta 4-isomers through the formation of an intermediate dienol. Mutation of the catalytic base (Asp-38) to Glu (D38E) has been found to reduce kcat/Km for the isomerization of 5-androstene-3,-17-dione (1) to 4-androstene-3,17-dione (3) by about 300-fold (Zawrotny et al., 1991). The free...

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PMID: 7947798
DOI: 10.1021/bi00250a044

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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