Article
Rapid detection of carbapenemase genes by multiplex real-time PCR.
The Journal of antimicrobial chemotherapy - 1 Apr 2012
Monteiro Jussimara, Widen Raymond H, Pignatari Antonio C C, Kubasek Carly, Silbert Suzane
Abstract excerpt
OBJECTIVES: To develop a single multiplex real-time PCR assay to detect six different genetic types of carbapenemases already identified in Enterobacteriaceae (KPC, GES, NDM, IMP, VIM and OXA-48). METHODS: A total of 58 bacterial isolates were tested. Thirty were previously characterized as resistant to carbapenems and documented by PCR and sequencing analysis to carry the following genes: bla(KPC) type, bla(GES)...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
