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Development of a set of multiplex PCR assays for the detection of genes encoding important β-lactamases in Enterobacteriaceae

12 Jan 2010

Abstract excerpt

OBJECTIVES: To develop a rapid and reliable tool to detect by multiplex PCR assays the most frequently widespread beta-lactamase genes encoding the OXA-1-like broad-spectrum beta-lactamases, extended-spectrum beta-lactamases (ESBLs), plasmid-mediated AmpC beta-lactamases and class A, B and D carbapenemases. METHODS: Following the design of a specific group of primers and optimization using control strains, a set...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 20071363
DOI: 10.1093/jac/dkp498

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