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Development and Evaluation of a Real-Time PCR Assay for Detection of <i>Klebsiella pneumoniae</i> Carbapenemase Genes

27 Nov 2008

Abstract excerpt

We developed a novel real-time PCR assay to detect Klebsiella pneumoniae carbapenemases (KPCs) and used this assay to screen clinical isolates of K. pneumoniae and Klebsiella oxytoca for the presence of bla(KPC) genes. The TaqMan real-time PCR assay amplified a 399-bp product from the bla(KPC) gene. The amplicon was designed so that the genes for isoenzymes KPC-1, -2, and -3 could be easily distinguished by...

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PMID: 19036932
DOI: 10.1128/jcm.01550-08

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