Article
MRE11 and RAD50, but not NBS1, are essential for gene targeting in the moss Physcomitrella patens.
Nucleic acids research - 1 Apr 2012
Kamisugi Yasuko, Schaefer Didier G, Kozak Jaroslav, Charlot Florence, Vrielynck Nathalie, Holá Marcela, Angelis Karel J, Cuming Andrew C, Nogué Fabien
Abstract excerpt
The moss Physcomitrella patens is unique among plant models for the high frequency with which targeted transgene insertion occurs via homologous recombination. Transgene integration is believed to utilize existing machinery for the detection and repair of DNA double-strand breaks (DSBs). We undertook targeted knockout of the Physcomitrella genes encoding components of the principal sensor of DNA DSBs, the MRN...
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