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Article

An evaluation of SNP-based PCR methods for the detection of β-lactamase-negative ampicillin-resistant Haemophilus influenzae.

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy - 1 Aug 2012

Witherden Elizabeth A, Kunde Dale, Tristram Stephen G

Abstract excerpt

Forty-four previously characterized strains of Haemophilus influenzae were used to evaluate the specificity of previously published SNP PCR primers for the detection of the N526K substitution in PBP3 of BLNAR isolates using real-time PCR. Hasegawa et al. primers that amplify strains without a substitution at 526 and fail to amplify strains with N526K were 100% sensitive and specific for detecting N526K. However,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 22203122
DOI: 10.1007/s10156-011-0356-5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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An evaluation of SNP-based PCR methods for the detection of β-lactamase-negative ampicillin-resistant Haemophilus influenzae.PMID 22203122Read ↗

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