Article
High expression of the penicillin G acylase gene (pac) from Bacillus megaterium UN1 in its own pac minus mutant.
Journal of applied microbiology - 1 Jul 2000
Panbangred W, Weeradechapon K, Udomvaraphant S, Fujiyama K, Meevootisom V
Abstract excerpt
By marker exchange mutagenesis, Bacillus megaterium strain UN-1 (Bm-UN1) was used to prepare a mutant strain B. megaterium UN-cat (Bm-UNcat) lacking the penicillin G acylase gene (pac). The pac gene from Bm-UN1 was subcloned into pTF6 and the resultant plasmid, pBA402, was introduced into Bm-UNcat and Bacillus subtilis. Bm-UNcat harbouring pBA402 produced high penicillin G acylase (PAC) activity of 13.7, 19.5 and...
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