Article
Translocation of ProOmpA possessing an intramolecular disulfide bridge into membrane vesicles of Escherichia coli. Effect of membrane energization.
The Journal of biological chemistry - 5 Oct 1990
Tani K, Tokuda H, Mizushima S
Abstract excerpt
In the absence of delta mu H+, the in vitro translocation of proOmpA resulted in the stable accumulation of a possible translocation intermediate in addition to a transiently accumulating one. The stable intermediate was detected on a polyacrylamide gel as two proteinase K-resistant bands corresp...
Topics
- Bacterial Outer Membrane Proteins
- Cell Membrane
- Chromatography, Gel
- Disulfides
- Escherichia coli
- Escherichia coli Proteins
- Ethylmaleimide
- Immunoblotting
- Mutation
- Plasmids
- Protein Biosynthesis
- Protein Precursors
- Protein Processing, Post-Translational
- Sulfur Radioisotopes
- Transcription, Genetic
- Tritium