Genes required for formation of the apoMoFe protein of Klebsiella pneumoniae nitrogenase in Escherichia coli.

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Article

Genes required for formation of the apoMoFe protein of Klebsiella pneumoniae nitrogenase in Escherichia coli.

The Journal of biological chemistry - 15 Sept 1990

Harris G S, White T C, Flory J E, Orme-Johnson W H

Abstract excerpt

A binary plasmid system was used to produce nitrogenase components in Escherichia coli and subsequently to define a minimum set of nitrogen fixation (nif) genes required for the production of the iron-molybdenum cofactor (FeMoco) reactivatable apomolybdenum-iron (apoMoFe) protein of nitrogenase. The active MoFe protein is an alpha 2 beta 2 tetramer containing two FeMoco clusters and 4 Fe4S4 P centers (for review...

Topics

Amino Acid Sequence
Apoproteins
Bacterial Proteins
Chromosome Deletion
Escherichia coli
Ferredoxins
Genes, Bacterial
Genotype
Klebsiella pneumoniae
Molecular Sequence Data
Molecular Weight

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molybdoferredoxin

Mutation

Nitrogen Fixation

Nitrogenase

Phenotype

Plasmids

Recombinant Proteins

Source

Open in PubMed

Identifiers

PMID: 2203791

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2203791-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Genes required for formation of the apoMoFe protein of Klebsiella pneumoniae nitrogenase in Escherichia coli.PMID 2203791Read ↗

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