Article
Apodinitrogenase: purification, association with a 20-kilodalton protein, and activation by the iron-molybdenum cofactor in the absence of dinitrogenase reductase.
Biochemistry - 10 Apr 1990
Paustian T D, Shah V K, Roberts G P
Abstract excerpt
The Azotobacter vinelandii mutant strain UW45 contains a mutation in the nifB gene and produces an inactive dinitrogenase protein that can be activated by the addition of purified iron-molybdenum cofactor (FeMoco). This FeMoco-deficient dinitrogenase (Apo I) has now been purified 96-fold to great...
Topics
- Apoenzymes
- Apoproteins
- Azotobacter
- Chromatography
- Chromatography, DEAE-Cellulose
- Chromatography, High Pressure Liquid
- Dinitrogenase Reductase
- Durapatite
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Enzyme Stability
- Ferredoxins
- Genes, Bacterial
- Hot Temperature
- Hydroxyapatites
- Kinetics
- Molecular Weight
- Molybdoferredoxin