Article
The product of the Klebsiella pneumoniae nifX gene is a negative regulator of the nitrogen fixation (nif) regulon.
Journal of bacteriology - 1 Mar 1990
Gosink M M, Franklin N M, Roberts G P
Abstract excerpt
An insertional mutation was made in the nifX gene of Klebsiella pneumoniae. This mutation had little effect on the nitrogenase activity of the strain, as measured by acetylene reduction. However, on the addition of NH4+ or O2 (conditions which block nif protein synthesis by transcriptional and po...
Topics
- Acetylene
- Bacterial Proteins
- Blotting, Northern
- Blotting, Southern
- DNA Transposable Elements
- Electrophoresis, Gel, Two-Dimensional
- Genes, Bacterial
- Genes, Regulator
- Kinetics
- Klebsiella pneumoniae
- Mutation
- Nitrogen Fixation
- Plasmids
- Regulatory Sequences, Nucleic Acid
- Restriction Mapping