From gene to phenotype in Drosophila and other organisms.

Abstract excerpt

The growing number of cloned eukaryotic genes lacking a defined or proven biological function poses a major challenge in 'reverse genetics'. A method is described here that permits efficient screening for new lesions in, or close to, genes corresponding to cloned DNA sequences of interest. The technique involves transposon mutagenesis, followed by screening of DNA isolated from a population of mutagenised...

Topics

• Animals
• Cloning, Molecular
• Drosophila
• Genes
• Phenotype
• Polymerase Chain Reaction