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Article

Restoration of hydrogenase activity in hydrogenase-negative strains of Escherichia coli by cloned DNA fragments from Chromatium vinosum and Proteus vulgaris.

Journal of general microbiology - 1 Jun 1990

Chaudhuri A, Krasna A I

Abstract excerpt

DNA fragments from Proteus vulgaris and Chromatium vinosum were isolated which restored hydrogenase activities in both hydA and hydB mutant strains of Escherichia coli. The hydA and hydB genes, which map near minute 59 of the genome map, 17 kb distant from each other, are not structural hydrogenase genes, but mutation in either of these genes leads to failure to synthesize any of the hydrogenase isoenzymes. The...

Topics

Blotting, Southern
Blotting, Western
Chromatium
Cloning, Molecular
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Hydrogenase
Isoelectric Focusing
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Proteus vulgaris

Restriction Mapping

Transformation, Bacterial

Source

Identifiers

PMID: 2200847
DOI: 10.1099/00221287-136-6-1153

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2200847-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Restoration of hydrogenase activity in hydrogenase-negative strains of Escherichia coli by cloned DNA fragments from Chromatium vinosum and Proteus vulgaris.PMID 2200847Read ↗

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