Article
Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.
Journal of bacteriology - 1 Jul 1994
Menon N K, Chatelus C Y, Dervartanian M, Wendt J C, Shanmugam K T, Peck H D, Przybyla A E
Abstract excerpt
The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in...
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