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Article

Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.

Journal of bacteriology - 1 Jul 1994

Menon N K, Chatelus C Y, Dervartanian M, Wendt J C, Shanmugam K T, Peck H D, Przybyla A E

Abstract excerpt

The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Mutational Analysis
Escherichia coli
Genes, Bacterial
Genetic Complementation Test
Hydrogenase
Molecular Sequence Data
Operon

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Sequence Analysis, DNA

Source

Open in PubMed Open full text

Identifiers

PMID: 8021226
DOI: 10.1128/jb.176.14.4416-4423.1994
PMCID: PMC205655

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8021226-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.PMID 8021226Read ↗

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