Article
Multilocus fragment typing and genetic structure of Cryptosporidium parvum Isolates from diarrheic preweaned calves in Spain.
Applied and environmental microbiology - 1 Nov 2011
Quílez Joaquín, Vergara-Castiblanco Claudia, Monteagudo Luis, Del Cacho Emilio, Sánchez-Acedo Caridad
Abstract excerpt
A collection of 140 Cryptosporidium parvum isolates previously analyzed by PCR-restriction fragment length polymorphism (PCR-RFLP) and sequence analyses of the small-subunit (SSU) rRNA and 60-kDa glycoprotein (GP60) genes was further characterized by multilocus fragment typing of six minisatellite (MSB and MS5) and microsatellite (ML1, ML2, TP14, and 5B12) loci. Isolates were collected from diarrheic preweaned...
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