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Optimization of a fragment size analysis tool for identification of Cryptosporidium species and Gp60 alleles infecting domestic ruminants.

Veterinary parasitology - 15 Oct 2014

Ramo Ana, Quílez Joaquín, Del Cacho Emilio, Sánchez-Acedo Caridad

Abstract excerpt

A capillary electrophoresis (CE)-based DNA fragment analysis tool was optimized to identify in a single capillary the most common Cryptosporidium species and Cryptosporidium parvum GP60 alleles infecting domestic ruminants. For this purpose, a panel of genomic DNA samples including six Cryptosporidium species (C. parvum, C. bovis, C. ryanae, C. andersoni, C. ubiquitum, and C. hominis) and 18 C. parvum GP60...

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PMID: 25224787
DOI: 10.1016/j.vetpar.2014.08.025

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