Article
Use of a site-directed triple mutant to trap intermediates: demonstration that the flavin C(4a)-thiol adduct and reduced flavin are kinetically competent intermediates in mercuric ion reductase.
Biochemistry - 20 Mar 1990
Miller S M, Massey V, Ballou D, Williams C H, Distefano M D, Moore M J, Walsh C T
Abstract excerpt
A mutant form of mercuric reductase, which has three of its four catalytically essential cysteine residues replaced by alanines (ACAA: Ala135Cys140Ala558Ala559), has been constructed and used for mechanistic investigations. With disruption of the Hg(II) binding site, the mutant enzyme is devoid of Hg(II) reductase activity. However, it appears to fold properly since it binds FAD normally and exhibits very tight...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.