Article
Molecular assay optimized by Taguchi experimental design method for venous thromboembolism investigation.
Molecular and cellular probes - 1 Jan 2000
Celani de Souza Helder Jose, Moyses Cinthia B, Pontes Fabrício J, Duarte Roberto N, Sanches da Silva Carlos Eduardo, Alberto Fernando Lopes, Ferreira Ubirajara R, Silva Messias Borges
Abstract excerpt
Two mutations - Factor V Leiden (1691G > A) and the 20210G > A on the Prothrombin gene - are key risk factors for a frequent and potentially fatal disorder called Venous Thromboembolism. These molecular alterations can be investigated using real-time Polymerase Chain Reaction (PCR) with Fluorescence Resonance Energy Transfer (FRET) probes and distinct DNA pools for both factors. The objective of this paper is to...
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