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Article

DNA profiling of spermatozoa by laser capture microdissection and low volume-PCR.

PloS one - 1 Jan 2011

Li Cai-xia, Han Jun-ping, Ren Wen-yan, Ji An-quan, Xu Xiu-lan, Hu Lan

Abstract excerpt

Genetic profiling of sperm from complex biological mixtures such as sexual assault casework samples requires isolation of a pure sperm population and the ability to analyze low abundant samples. Current standard procedure for sperm isolation includes preferential lysis of epithelial contaminants followed by collection of intact sperm by centrifugation. While effective for samples where sperm are abundant, this...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 21853031
DOI: 10.1371/journal.pone.0022316
PMCID: PMC3154896

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