Article
Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides.
Forensic science international - 14 Oct 2003
Elliott K, Hill D S, Lambert C, Burroughes T R, Gill P
Abstract excerpt
Traditionally, sperms are isolated from vaginal cell mixtures by preferential extraction methods. Although these methods work well when there is a reasonable amount of DNA present, they are problematic when there are limited amounts (ca. 250 pg). In particular, the analysis of sperm from microscope slides has proven difficult. Here, we describe the use of laser capture microdissection (LM) for the isolation of...
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