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Article

Advantages of filtration method for sperm-DNA genotyping in sexual assault cases.

Legal medicine (Tokyo, Japan) - 1 Feb 2022

Nakagawa Toshifumi, Doi Masanori, Nishi Kosuke, Sugahara Takuya

Abstract excerpt

Differential extraction (DE) is a conventional method to isolate sperms from forensic semen samples (e.g. vaginal swab containing semen) for sperm-DNA genotyping. Subsequent to selective digestion of somatic cells in a mixture sample, sperms are collected and purified as a pellet by repetitive centrifugation based on the specific gravity of sperm heads. However, the centrifugation operation requires a technical...

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PMID: 34915337
DOI: 10.1016/j.legalmed.2021.101988

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