Cloning of the ethidium efflux gene from Escherichia coli.

doi:10.1016/0378-1097(90)90127-c

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Article

Cloning of the ethidium efflux gene from Escherichia coli.

FEMS microbiology letters - 1 Mar 1990

Purewal A S, Jones I G, Midgley M

Abstract excerpt

The gene specifying the ethidium efflux system of Escherichia coli has been cloned on a 3.2 kbp HindIII fragment and located on a 1.2 kbp fragment within this. Cross-resistance studies indicate that the system has a broad specificity for monovalent cations and the gene shows no hybridisation with...

Topics

Blotting, Southern
Cloning, Molecular
Drug Resistance, Microbial
Escherichia coli
Ethidium
Genes, Bacterial
Mutation
Plasmids
Restriction Mapping
Species Specificity
Staphylococcus

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