Detection of pyrimethamine resistance in Plasmodium falciparum by mutation-specific polymerase chain reaction.

doi:10.1016/0166-6851(90)90064-s

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Article

Detection of pyrimethamine resistance in Plasmodium falciparum by mutation-specific polymerase chain reaction.

Molecular and biochemical parasitology - 1 Mar 1990

Zolg J W, Chen G X, Plitt J R

Abstract excerpt

The point mutation at nucleotide 323 within the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum, which distinguishes pyrimethamine-sensitive from drug-resistant isolates, can be discriminated by the polymerase chain reaction using mutation-specific primers. The techniqu...

Topics

Animals
Base Sequence
Blotting, Southern
DNA
Drug Resistance
Genes
Molecular Sequence Data
Mutation
Plasmodium falciparum
Polymerase Chain Reaction
Pyrimethamine
Tetrahydrofolate Dehydrogenase
Thymidylate Synthase

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