Article
Detection of pyrimethamine resistance in Plasmodium falciparum by mutation-specific polymerase chain reaction.
Molecular and biochemical parasitology - 1 Mar 1990
Zolg J W, Chen G X, Plitt J R
Abstract excerpt
The point mutation at nucleotide 323 within the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum, which distinguishes pyrimethamine-sensitive from drug-resistant isolates, can be discriminated by the polymerase chain reaction using mutation-specific primers. The technique makes use of the principle that short oligonucleotides with a perfect match at their 3' ends, complementary to the...
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