Article
Characterization of a Yersinia enterocolitica biotype 1A strain harbouring an ail gene.
Journal of applied microbiology - 1 Oct 2011
Kraushaar B, Dieckmann R, Wittwer M, Knabner D, Konietzny A, Mäde D, Strauch E
Abstract excerpt
AIMS: The chromosomal ail gene (attachment and invasion locus) is commonly used as target gene for the detection of pathogenic Y. enterocolitica strains in food testing. The ail PCR does not detect strains of biotype 1A (BT1A), which are regarded as non-pathogenic because BT1A strains lack the virulence plasmid and chromosomally encoded virulence genes. In some recent reports, however, BT1A strains were...
Topics
- Bacterial Outer Membrane Proteins
- Cluster Analysis
- DNA, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Genes, Bacterial
- Genotype
- Plasmids
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization