Article
MDCK cell line with inducible allele B NS1 expression propagates delNS1 influenza virus to high titres.
Vaccine - 16 Sept 2011
van Wielink R, Harmsen M M, Martens D E, Peeters B P H, Wijffels R H, Moormann R J M
Abstract excerpt
Influenza A viruses lacking the gene encoding the non-structural NS1 protein (delNS1) have potential use as live attenuated vaccines. However, due to the lack of NS1, virus replication in cell culture is considerably reduced, prohibiting commercial vaccine production. We therefore established two stable MDCK cell lines that show inducible expression of the allele B NS1 protein. Upon induction, both cell lines...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.