Article
Rapid detection of the H275Y oseltamivir resistance mutation in influenza A/H1N1 2009 by single base pair RT-PCR and high-resolution melting.
PloS one - 1 Jan 2011
Tong Steven Y C, Dakh Farshid, Hurt Aeron C, Deng Yi-Mo, Freeman Kevin, Fagan Peter K, Barr Ian G, Giffard Philip M
Abstract excerpt
INTRODUCTION: We aimed to design a real-time reverse-transcriptase-PCR (rRT-PCR), high-resolution melting (HRM) assay to detect the H275Y mutation that confers oseltamivir resistance in influenza A/H1N1 2009 viruses. FINDINGS: A novel strategy of amplifying a single base pair, the relevant SNP at position 823 of the neuraminidase gene, was chosen to maintain specificity of the assay. Wildtype and mutant virus...
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