Article
Effects of mechanical strain on ANK, ENPP1 and TGF-β1 expression in rat endplate chondrocytes in vitro.
Molecular medicine reports - 1 Jan 2000
Xu Hong-Guang, Hu Chun-Jiang, Wang Hong, Liu Ping, Yang Xiao-Ming, Zhang Yu, Wang Ling-Ting
Abstract excerpt
We investigated the effects of mechanical strain on the progressive ankylosis (ANK) gene and extracellular nucleotide phosphatase/phosphodiesterase (ENPP)1 mRNA expression and TGF-β1 protein expression in rat endplate chondrocytes in vitro. Endplate chondrocytes were isolated and cultured in vitro. Following identification with toluidine blue and immunocytochemical staining, chondrocytes were subjected to 10%...
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