Article
High-resolution amplified fragment length polymorphism typing of Lactococcus lactis strains enables identification of genetic markers for subspecies-related phenotypes.
Applied and environmental microbiology - 1 Aug 2011
Kütahya Oylum Erkus, Starrenburg Marjo J C, Rademaker Jan L W, Klaassen Corné H W, van Hylckama Vlieg Johan E T, Smid Eddy J, Kleerebezem Michiel
Abstract excerpt
A high-resolution amplified fragment length polymorphism (AFLP) methodology was developed to achieve the delineation of closely related Lactococcus lactis strains. The differentiation depth of 24 enzyme-primer-nucleotide combinations was experimentally evaluated to maximize the number of polymorphisms. The resolution depth was confirmed by performing diversity analysis on 82 L. lactis strains, including both...
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