Article
Generation of collagenase-resistant collagen by site-directed mutagenesis of murine pro alpha 1(I) collagen gene.
Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1990
Wu H, Byrne M H, Stacey A, Goldring M B, Birkhead J R, Jaenisch R, Krane S M
Abstract excerpt
Collagenase (matrix metalloproteinase 1) cleaves type I, II, and III collagen helices at a specific site between Gly-Ile or Gly-Leu bonds (residues 775 and 776, P1-P1'). To understand the mechanism of collagen processing, mutations around the cleavage site have been introduced into the cloned mur...
Topics
- Amino Acid Sequence
- Animals
- Arthritis, Rheumatoid
- Base Sequence
- Cells, Cultured
- Clone Cells
- Collagen
- Fibroblasts
- Genes
- Humans
- Macromolecular Substances
- Mice
- Microbial Collagenase
- Molecular Sequence Data
- Mutation