Article
Amino acid sequence requirements for the association of apocytochrome c with mitochondria.
Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1990
Sprinkle J R, Hakvoort T B, Koshy T I, Miller D D, Margoliash E
Abstract excerpt
To examine the amino acid sequence requirements for the biphasic association of Drosophila melanogaster apocytochrome c with mouse liver mitochondria in vitro, recombinant constructs of the protein were prepared. Removal of the C-terminal sequence to residue 58 had little influence, but truncation to residue 50 decreased the association to low levels and removal to residue 36 was even more effective. However, a...
Topics
- Amino Acid Sequence
- Animals
- Apoproteins
- Chromosome Deletion
- Cloning, Molecular
- Cytochrome c Group
- Cytochromes c
- Drosophila melanogaster
- Kinetics
- Mice
- Mitochondria, Liver