Article
[Screening of high lipid production Trichosporon fermentans mutants by transposon tagging mTn-lacZ/leu2 insertion].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Mar 2011
Song Andong, Liu Yubo, Xie Hui, Wang Fengqin, Bao Xiaoming
Abstract excerpt
To improve microbial lipid production, we inserted mTn-lacZ/leu2 into Trichosporon fermentans 2.1368-Leu(-) to obtain high lipid production mutants. By observing the LacZ chromogenic change, the positive reaction between Cerulenin (inhibitor of fatty acid synthase) and phosphate vanillin, a higher lipid-producing mutant 2.1368-Leu(-)-7 grown on corn-stalk hydrolysate was obtained. The lipid content of this mutant...
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