Article
Rapid identification of Lactobacillus plantarum group using the SNaPshot minisequencing assay.
Systematic and applied microbiology - 1 Dec 2011
Huang Chien-Hsun, Chang Mu-Tzu, Huang Mu-Chiou, Lee Fwu-Ling
Abstract excerpt
This study used SNaPshot minisequencing for species identification within the Lactobacillus plantarum group. A SNaPshot minisequencing assay using dnaK as a target gene was developed, and five SNP primers were designed by analysing the conserved regions of the dnaK sequences. The specificity of the minisequencing assay was evaluated using 35 strains of L. plantarum group species. The results showed that the...
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