Article
Application of the SNaPshot minisequencing assay to species identification in the Lactobacillus casei group.
Molecular and cellular probes - 1 Aug 2011
Huang Chien-Hsun, Chang Mu-Tzu, Huang Mu-Chiou, Lee Fwu-Ling
Abstract excerpt
This study used group-specific PCR combined with SNaPshot minisequencing for species identification within the Lactobacillus casei group. The L. casei group-specific PCR primer pair was designed using the rpoA gene sequence. A SNaPshot minisequencing assay using dnaK as a target gene was developed, and five SNP primers were designed by analysing the conserved regions of the dnaK sequences. The specificity of the...
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