Article
Discrimination of the Lactobacillus acidophilus group using sequencing, species-specific PCR and SNaPshot mini-sequencing technology based on the recA gene.
Journal of the science of food and agriculture - 1 Oct 2012
Huang Chien-Hsun, Chang Mu-Tzu, Huang Mu-Chiou, Wang Li-Tin, Huang Lina, Lee Fwu-Ling
Abstract excerpt
BACKGROUND: To clearly identify specific species and subspecies of the Lactobacillus acidophilus group using phenotypic and genotypic (16S rDNA sequence analysis) techniques alone is difficult. The aim of this study was to use the recA gene for species discrimination in the L. acidophilus group, as well as to develop a species-specific primer and single nucleotide polymorphism primer based on the recA gene...
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