Article
Synthesis of a Klebsiella pneumoniae O-antigen heteropolysaccharide (O12) requires an ABC 2 transporter.
Journal of bacteriology - 1 Mar 2003
Izquierdo Luis, Merino Susana, Regué Miguel, Rodriguez Florencia, Tomás Juan M
Abstract excerpt
A recombinant clone encoding enzymes for Klebsiella pneumoniae O12-antigen lipopolysaccharide (LPS) was found when we screened for serum resistance of a cosmid-based genomic library of K. pneumoniae KT776 (O12:K80) introduced into Escherichia coli DH5alpha. A total of eight open reading frames (ORFs) (wb(O12) gene cluster) were necessary to produce K. pneumoniae O12-antigen LPS in E. coli K-12. A complete...
Topics
- ATP-Binding Cassette Transporters
- Amino Acid Sequence
- Bacterial Proteins
- Cloning, Molecular
- Escherichia coli
- Genetic Complementation Test
- Klebsiella pneumoniae
- Molecular Sequence Data
- Multigene Family
- Mutation
- O Antigens
- Sequence Analysis, Protein
- Sequence Homology, Nucleic Acid