Article
Direct sequencing of the amplified structural gene and promoter for the extended-broad-spectrum beta-lactamase TEM-9 (RHH-1) of Klebsiella pneumoniae.
Plasmid - 1 Jan 1990
Mabilat C, Goussard S, Sougakoff W, Spencer R C, Courvalin P
Abstract excerpt
Extended-broad-spectrum beta-lactamase TEM-9, detected in a clinical isolate of Klebsiella pneumoniae, confers high-level resistance to recent cephalosporins, in particular ceftazidime, and to the monobactam aztreonam. Using oligonucleotide probes, we found that the plasmid gene blaT-9 encoding TEM-9 differs from characterized blaT genes by a new combination of already known mutations. Gene blaT-9 was further...
Topics
- Amino Acids
- Base Sequence
- DNA Transposable Elements
- DNA, Bacterial
- Genes, Bacterial
- Klebsiella pneumoniae
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Promoter Regions, Genetic