Article
High-throughput SNP discovery and genotyping in durum wheat (Triticum durum Desf.).
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Aug 2011
Trebbi Daniele, Maccaferri Marco, de Heer Peter, Sørensen Anker, Giuliani Silvia, Salvi Silvio, Sanguineti Maria Corinna, Massi Andrea, van der Vossen Edwin Andries Gerard, Tuberosa Roberto
Abstract excerpt
We describe the application of complexity reduction of polymorphic sequences (CRoPS(®)) technology for the discovery of SNP markers in tetraploid durum wheat (Triticum durum Desf.). A next-generation sequencing experiment was carried out on reduced representation libraries obtained from four durum cultivars. SNP validation and minor allele frequency (MAF) estimate were carried out on a panel of 12 cultivars, and...
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