Uniformity of the Splicing Pattern of the E6/E7 Transcripts in Human Papillomavirus Type 16-transformed Human Fibroblasts, Human Cervical Premalignant Lesions and Carcinomas

Abstract excerpt

We utilized the RNA polymerase chain reaction (PCR) to analyse the transcripts of the E6/E7 open reading frames of human papillomavirus type 16 (HPV-16). Total RNA was isolated from 14 cervical squamous carcinomas, nine cervical intraepithelial neoplasias and from human fibroblasts transformed with different HPV-16 constructs. In all specimens two spliced transcripts were detected. Sequence analysis of the cloned...

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