Article
Conditional mutations in OutE and OutL block exoenzyme secretion across the Erwinia carotovora outer membrane.
FEMS microbiology letters - 1 Aug 1998
Housby J N, Thomas J D, Wharam S D, Reeves P J, Salmond G P
Abstract excerpt
The phytopathogen Erwinia carotovora subspecies carotovora secretes pectinases and cellulase via the general secretory pathway, a process requiring at least 13 proteins encoded by the out gene cluster. By exploiting delta::Tn5, a generalised transducing phage (psi KP) and localised mutagenesis of the out gene cluster, we have produced a histidine auxotroph and 19 new secretory mutants, including two (HJN1003 and...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
