Conditional mutations in OutE and OutL block exoenzyme secretion across the Erwinia carotovora outer membrane.

doi:10.1111/j.1574-6968.1998.tb13132.x

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Article

Conditional mutations in OutE and OutL block exoenzyme secretion across the Erwinia carotovora outer membrane.

FEMS microbiology letters - 1 Aug 1998

Housby J N, Thomas J D, Wharam S D, Reeves P J, Salmond G P

Abstract excerpt

The phytopathogen Erwinia carotovora subspecies carotovora secretes pectinases and cellulase via the general secretory pathway, a process requiring at least 13 proteins encoded by the out gene cluster. By exploiting delta::Tn5, a generalised transducing phage (psi KP) and localised mutagenesis of...

Topics

Bacterial Outer Membrane Proteins
Bacteriophages
Cellulase
Cloning, Molecular
Genes, Bacterial
Mutagenesis
Pectobacterium carotovorum
Phenotype
Polygalacturonase
Polymerase Chain Reaction
Temperature

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