Article
Rapid and simultaneous detection of Mycobacterium tuberculosis complex and Beijing/W genotype in sputum by an optimized DNA extraction protocol and a novel multiplex real-time PCR.
Journal of clinical microbiology - 1 Jul 2011
Leung Eric T Y, Zheng L, Wong Rity Y K, Chan Edward W C, Au T K, Chan Raphael C Y, Lui Grace, Lee Nelson, Ip Margaret
Abstract excerpt
Rapid diagnosis and genotyping of Mycobacterium tuberculosis by molecular methods are often limited by the amount and purity of DNA extracted from body fluids. In this study, we evaluated 12 DNA extraction methods and developed a highly sensitive protocol for mycobacterial DNA extraction directly from sputa using surface-coated magnetic particles. We have also developed a novel multiplex real-time PCR for...
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