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Development of a One-Step Multiplex PCR Assay for Differential Detection of Major Mycobacterium Species

29 Jun 2017

Abstract excerpt

ABSTRACT The prevalence of tuberculosis continues to be high, and nontuberculous mycobacterial (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whole-genome analysis and bioinformatics to identify novel molecular targets. The aims of...

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PMID: 28659320
DOI: 10.1128/jcm.00549-17

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