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Article

Real-time PCR assay for rapid detection of epidemiologically and clinically significant Mycobacterium tuberculosis Beijing genotype isolates.

Journal of clinical microbiology - 1 May 2014

Mokrousov Igor, Vyazovaya Anna, Zhuravlev Viacheslav, Otten Tatiana, Millet Julie, Jiao Wei-Wei, Shen A-Dong, Rastogi Nalin, Vishnevsky Boris, Narvskaya Olga

Abstract excerpt

Mycobacterium tuberculosis Beijing genotype strains are rapidly disseminating, frequently hypervirulent, and multidrug resistant. Here, we describe a method for their rapid detection by real-time PCR that targets the specific IS6110 insertion in the dnaA-dnaN genome region. The method was evaluated with a geographically and genetically diverse collection representing areas in East Asia and the former Soviet Union...

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Identifiers

PMID: 24523461
DOI: 10.1128/JCM.03193-13
PMCID: PMC3993684

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